| Checkpoint | Distance from start |
|---|---|
| Anchorage | 0 |
| Campbel Airstrip (BLM) | 11 |
| Ruby | 328 |
| Galena | 380 |
| Nulato | 432 |
| Kaltag 1 | 474 |
| Eagle Island 1 | 544 |
| Grayling 1 | 604 |
| Anvik | 622 |
| Grayling 2 | 640 |
| Eagle Island 2 | 700 |
| Kaltag 2 | 770 |
| Unalakleet | 860 |
| Shaktoolik | 902 |
| Koyuk | 950 |
| Elim | 998 |
| White Mountain | 1044 |
| Safety | 1099 |
| Nome | 1121 |
