| Checkpoint | Distance from start |
|---|---|
| Anchorage | 0 |
| Campbell Airstrip (BLM) | 11 |
| Campbel Airstrip (BLM) | 11 |
| Willow | 70 |
| Yentna Station | 115 |
| Yentna (YENT-na) | 115 |
| Skwentna | 149 |
| Finger Lake | 194 |
| Rainy Pass | 224 |
| Rohn (Rone) | 272 |
| Nikolai (NIK-o-lye) | 352 |
| McGrath | 400 |
| Takotna (Ta-COT-na) | 418 |
| Ophir (OH-fur) | 443 |
| Iditarod (I-DIT-a-rod) | 533 |
| Shageluk (SHAG-a-luck) | 598 |
| Anvik | 623 |
| Grayling | 641 |
| Eagle Island | 701 |
| Kaltag (KAL-tag) | 771 |
| Unalakleet (YOU-na-la-kleet) | 861 |
| Shaktoolik (Shak-TOO-lick) | 903 |
| Koyuk (Coy-uk) | 951 |
| Elim (EE-lum) | 999 |
| White Mountain | 1045 |
| Safety | 1100 |
| Nome | 1122 |
