| Checkpoint | Distance from start |
|---|---|
| Anchorage | 0 |
| Campbell Airstrip | 20 |
| Willow | 49 |
| Yentna | 115 |
| Skwentna | 149 |
| Finger Lake | 194 |
| Rainy Pass | 224 |
| Rohn | 272 |
| Nikolai | 347 |
| McGrath | 401 |
| Takotna | 419 |
| Ophir | 444 |
| Iditarod | 534 |
| Shageluk | 599 |
| Anvik | 624 |
| Grayling | 642 |
| Eagle Island | 702 |
| Kaltag | 772 |
| Unalakleet | 862 |
| Shaktoolik | 902 |
| Koyuk | 960 |
| Elim | 1008 |
| White Mountain | 1054 |
| Safety | 1109 |
| Nome | 1131 |
